fla st standard Search Results


fla st standard  (InvivoGen)
94
InvivoGen fla st standard
Fla St Standard, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fla st standard/product/InvivoGen
Average 94 stars, based on 1 article reviews
fla st standard - by Bioz Stars, 2026-03
94/100 stars
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flagellin  (InvivoGen)
96
InvivoGen flagellin
PF-04620110 suppresses fatty acid-induced nucleotide-binding domain, leucine-rich-repeat-containing receptor (NLR), pyrin-domain-containing 3 (NLRP3) inflammasome activation. (A) Quantification of interleukin 1β (IL-1β; left), IL-18 (middle), and tumor necrosis factor α (TNF-α; right) secretion from wild-type (WT) bone marrow-derived macrophages (BMDMs) were pretreated with PF-04620110 (50 µM, 2 hours) or dimethyl sulfoxide (DMSO) (control), followed by incubation with palmitate-bovine serum albumin (PA-BSA) after lipopolysaccharide (LPS) stimulation ( n =10 mice per group). (B) Quantification of IL-1β secretion from WT BMDMs that were pretreated with PF-04620110 in a dose-dependent manner (12.5, 25, 50, or 100 µM, 2 hours) or DMSO (control), followed by incubation with PA-BSA after LPS stimulation ( n =10 mice per group). (C) Quantification of IL-1β secretion from WT BMDMs that were pretreated with PF-04620110 (50 µM, 2 hours) or DMSO (control), followed by incubation with poly(dA:dT) or <t>flagellin</t> after LPS stimulation ( n =10 mice per group). (D) Representative immunoblot analysis for caspase-1 and IL-1β (left), and densitometry quantification of caspase-1 p10 and IL-1β p17 levels (normalized to levels of β-actin) (right), from WT BMDMs that were pretreated with PF-04620110 (50 µM, 2 hours) or DMSO, followed by incubation with PA-BSA after LPS stimulation. For immunoblots, β-actin was used as loading control ( n =6 mice per group). Data are mean±standard deviation. a P <0.001, b P <0.01, c P <0.05 by two-tailed t -test or analysis of variance.
Flagellin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flagellin/product/InvivoGen
Average 96 stars, based on 1 article reviews
flagellin - by Bioz Stars, 2026-03
96/100 stars
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astm d93 flash point standard approx value  (VHG Labs)
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ASTM D93 Flash Point Standard Approx Value 27°C
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taqman array mouse inflammation 96 well plate standard configurable  (Thermo Fisher)
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The Inflammation Panel targets genes implicated in a range of inflammatory diseases
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taqman array mouse pain neuropathic inflammatory 96 well plate standard configurable  (Thermo Fisher)
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The Pain Neuropathic Inflammatory array covers genes involved in the transduction maintenance and modulation of pain responses Included are the complex of activities related to the neurotransmission of damage sensing neurons nociceptors and the associated
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flame photometer std caesium 1000 ppm can be made  (Reagecon)
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Flame Photometer Std Caesium 1000 ppm can be made
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macrophage inflammatory protein 4 ccl18 nm 002988 human mass spec standard  (OriGene)
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CCL18 MS Standard C13 and N15 labeled recombinant protein NP 002979
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rooibos flavonoid standards kit  (ChromaDex)
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Rooibos Flavonoid Standards Kit SH
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soy isoflavone standards kit  (ChromaDex)
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Soy Isoflavone Standards Kit AS
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black tea theaflavin standards kit  (ChromaDex)
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Black Tea Theaflavin Standards Kit P
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Image Search Results


PF-04620110 suppresses fatty acid-induced nucleotide-binding domain, leucine-rich-repeat-containing receptor (NLR), pyrin-domain-containing 3 (NLRP3) inflammasome activation. (A) Quantification of interleukin 1β (IL-1β; left), IL-18 (middle), and tumor necrosis factor α (TNF-α; right) secretion from wild-type (WT) bone marrow-derived macrophages (BMDMs) were pretreated with PF-04620110 (50 µM, 2 hours) or dimethyl sulfoxide (DMSO) (control), followed by incubation with palmitate-bovine serum albumin (PA-BSA) after lipopolysaccharide (LPS) stimulation ( n =10 mice per group). (B) Quantification of IL-1β secretion from WT BMDMs that were pretreated with PF-04620110 in a dose-dependent manner (12.5, 25, 50, or 100 µM, 2 hours) or DMSO (control), followed by incubation with PA-BSA after LPS stimulation ( n =10 mice per group). (C) Quantification of IL-1β secretion from WT BMDMs that were pretreated with PF-04620110 (50 µM, 2 hours) or DMSO (control), followed by incubation with poly(dA:dT) or flagellin after LPS stimulation ( n =10 mice per group). (D) Representative immunoblot analysis for caspase-1 and IL-1β (left), and densitometry quantification of caspase-1 p10 and IL-1β p17 levels (normalized to levels of β-actin) (right), from WT BMDMs that were pretreated with PF-04620110 (50 µM, 2 hours) or DMSO, followed by incubation with PA-BSA after LPS stimulation. For immunoblots, β-actin was used as loading control ( n =6 mice per group). Data are mean±standard deviation. a P <0.001, b P <0.01, c P <0.05 by two-tailed t -test or analysis of variance.

Journal: Diabetes & Metabolism Journal

Article Title: PF-04620110, a Potent Antidiabetic Agent, Suppresses Fatty Acid-Induced NLRP3 Inflammasome Activation in Macrophages

doi: 10.4093/dmj.2019.0112

Figure Lengend Snippet: PF-04620110 suppresses fatty acid-induced nucleotide-binding domain, leucine-rich-repeat-containing receptor (NLR), pyrin-domain-containing 3 (NLRP3) inflammasome activation. (A) Quantification of interleukin 1β (IL-1β; left), IL-18 (middle), and tumor necrosis factor α (TNF-α; right) secretion from wild-type (WT) bone marrow-derived macrophages (BMDMs) were pretreated with PF-04620110 (50 µM, 2 hours) or dimethyl sulfoxide (DMSO) (control), followed by incubation with palmitate-bovine serum albumin (PA-BSA) after lipopolysaccharide (LPS) stimulation ( n =10 mice per group). (B) Quantification of IL-1β secretion from WT BMDMs that were pretreated with PF-04620110 in a dose-dependent manner (12.5, 25, 50, or 100 µM, 2 hours) or DMSO (control), followed by incubation with PA-BSA after LPS stimulation ( n =10 mice per group). (C) Quantification of IL-1β secretion from WT BMDMs that were pretreated with PF-04620110 (50 µM, 2 hours) or DMSO (control), followed by incubation with poly(dA:dT) or flagellin after LPS stimulation ( n =10 mice per group). (D) Representative immunoblot analysis for caspase-1 and IL-1β (left), and densitometry quantification of caspase-1 p10 and IL-1β p17 levels (normalized to levels of β-actin) (right), from WT BMDMs that were pretreated with PF-04620110 (50 µM, 2 hours) or DMSO, followed by incubation with PA-BSA after LPS stimulation. For immunoblots, β-actin was used as loading control ( n =6 mice per group). Data are mean±standard deviation. a P <0.001, b P <0.01, c P <0.05 by two-tailed t -test or analysis of variance.

Article Snippet: Lipopolysaccharide (LPS) ( Escherichia coli ; tlrl-pelps) and flagellin ( Salmonella typhimurium ; tlrl-stfla) were from Invivogen (San Diego, CA, USA).

Techniques: Binding Assay, Activation Assay, Derivative Assay, Incubation, Western Blot, Standard Deviation, Two Tailed Test

Genetic inhibition of diacylglycerol acyltransferase-1 (DGAT1) suppresses fatty acid-induced nucleotide-binding domain, leucine-rich-repeat-containing receptor (NLR), pyrin-domain-containing 3 (NLRP3) inflammasome activation. (A) Representative immunoblot analysis for DGAT1, caspase-1, and interleukin 1β (IL-1β; left), and densitometry quantification of DGAT1, caspase-1 p10, and IL-1β p17 levels (normalized to levels of β-actin; right), from wild-type (WT) bone marrow-derived macrophages (BMDMs) were transduced with two independent Dgat1 -targeting gRNAs ( Dgat1 gRNA #1 and Dgat1 gRNA #2), or with a control plasmid (control), and were stimulated with lipopolysaccharide (LPS) and palmitate-bovine serum albumin (PA-BSA). For immunoblots, β-actin was used as loading control ( n =5 mice per group). (B) Quantification of IL-1β (left), IL-18 (middle), and tumor necrosis factor α (TNF-α; right) secretion from WT BMDMs were transduced with two independent Dgat1 -targeting gRNAs ( Dgat1 gRNA #1 and Dgat1 gRNA #2), or with a control plasmid (control), and were stimulated with LPS and PA-BSA ( n =10 mice per group). (C) Quantification of IL-1β and IL-18 secretion from WT BMDMs that were transduced with two independent Dgat1 -targeting gRNAs ( Dgat1 gRNA #1 and Dgat1 gRNA #2), or with a control plasmid (control), and were incubated with poly(dA:dT) or flagellin after LPS stimulation ( n =10 mice per group). Data are mean±standard deviation. a P <0.01, b P <0.05, by two-tailed t -test or analysis of variance.

Journal: Diabetes & Metabolism Journal

Article Title: PF-04620110, a Potent Antidiabetic Agent, Suppresses Fatty Acid-Induced NLRP3 Inflammasome Activation in Macrophages

doi: 10.4093/dmj.2019.0112

Figure Lengend Snippet: Genetic inhibition of diacylglycerol acyltransferase-1 (DGAT1) suppresses fatty acid-induced nucleotide-binding domain, leucine-rich-repeat-containing receptor (NLR), pyrin-domain-containing 3 (NLRP3) inflammasome activation. (A) Representative immunoblot analysis for DGAT1, caspase-1, and interleukin 1β (IL-1β; left), and densitometry quantification of DGAT1, caspase-1 p10, and IL-1β p17 levels (normalized to levels of β-actin; right), from wild-type (WT) bone marrow-derived macrophages (BMDMs) were transduced with two independent Dgat1 -targeting gRNAs ( Dgat1 gRNA #1 and Dgat1 gRNA #2), or with a control plasmid (control), and were stimulated with lipopolysaccharide (LPS) and palmitate-bovine serum albumin (PA-BSA). For immunoblots, β-actin was used as loading control ( n =5 mice per group). (B) Quantification of IL-1β (left), IL-18 (middle), and tumor necrosis factor α (TNF-α; right) secretion from WT BMDMs were transduced with two independent Dgat1 -targeting gRNAs ( Dgat1 gRNA #1 and Dgat1 gRNA #2), or with a control plasmid (control), and were stimulated with LPS and PA-BSA ( n =10 mice per group). (C) Quantification of IL-1β and IL-18 secretion from WT BMDMs that were transduced with two independent Dgat1 -targeting gRNAs ( Dgat1 gRNA #1 and Dgat1 gRNA #2), or with a control plasmid (control), and were incubated with poly(dA:dT) or flagellin after LPS stimulation ( n =10 mice per group). Data are mean±standard deviation. a P <0.01, b P <0.05, by two-tailed t -test or analysis of variance.

Article Snippet: Lipopolysaccharide (LPS) ( Escherichia coli ; tlrl-pelps) and flagellin ( Salmonella typhimurium ; tlrl-stfla) were from Invivogen (San Diego, CA, USA).

Techniques: Inhibition, Binding Assay, Activation Assay, Western Blot, Derivative Assay, Transduction, Plasmid Preparation, Incubation, Standard Deviation, Two Tailed Test